Spanish health authorities said on Wednesday that another person being monitored in Madrid for Ebola had tested negative for the disease.
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The man, a Spaniard who had traveled from Nigeria, was one of several people hospitalized after authorities confirmed on Monday that a Spanish nurse had caught the disease in Madrid.
A second nurse was also cleared of Ebola. A third nursing assistant was hospitalised late on Tuesday for monitoring, a source at La Paz hospital said - bringing the number of people examined in hospital for Ebola to five, two of whom tested negative.
The first case of Ebola being contracted outside of West Africa was reported in Spain, and the World Health Organization expects more cases in Europe.
Spanish priest Miguel Pajares, 75, the first European infected by a strain of Ebola that has spread in West Africa died in a Madrid hospital in August after contracting the disease while working for a nonprofit in Liberia.
In Dallas meanwhile, the Ebola patient fighting for his life in hospital was on a ventilator and a kidney dialysis machine to help stabilize his health, the hospital said Tuesday.
Liberian national Thomas Eric Duncan, the first person diagnosed with the deadly virus on U.S. soil, has also been given the experimental medication brincidofovir.
A hospital in Nebraska said it is using the same drug to treat an American journalist who was airlifted from Liberia and arrived Monday.
Authorities in the United States and the public are on alert following Duncan's diagnosis more than a week ago, which raised concerns that the worst epidemic of Ebola on record could spread from three hardest-hit impoverished countries - Guinea, Sierra Leone and Liberia. Duncan, who arrived in Texas in late September on a commercial flight from Liberia, has been in critical condition since Saturday.