This will hurt the system. At off peak times, the system will have to add a mens and women's car when both would be mostly empty. The choice will be to operate a lot of empty cars or not to run the line at all, and the decision will undoubtedly be to not run the line. Also running the line on Saturday will be an area of contention. If one could reson with them, one could propose kosher cars in exchange for not objecting to running on Saturday, but they are absolutists and not amenable to compromise, so what is the point.
Malaysia sends team to Reunion Island to see if aircraft debris is from MH370 flight (Reuters)
from the article: Jerusalem light rail may have segregated men-only / women-only cars